HOME-MADE Kb MARKERS

Heidi Moss hmmoss at MAIL.MED.CORNELL.EDU
Mon Feb 6 22:04:26 EST 1995


Our lab is spending a fortune on manufactured Kb ladder -- does anyone 
have any good suggestions for making our own? We want one for both high 
M.W. (1-20kb), and low M.W. (0-1kb). I know lambda digested with HindIII is 
one possibility (where can one get PLAIN, W.T. lambda phage? what e-coli 
strain is best for their propagation?), but any other suggestions would 
be greatly appreciated. 
THANKS!!
-- Heidi



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