Sequencing from PCR, gel problem?

BOURDEAU,ANNIE,MS B2QK000 at MUSICB.MCGILL.CA
Tue Feb 7 09:48:16 EST 1995


Hi!

    Using the Pharmacia T7 sequencing kit, I am sequencing from purified
and precipitated PCR reactions. When those reactions are run on a 6% Bis
Acrylamide gel, there is no visible reaction on the long run, and
sequence can be read only in the upper part of the short run. The bands
do not seem to migrate properly.
    Is it the gel? or is it something else that makes the sequencing
reading very short (about 130 bases)?

      Thank you, Annie




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