library purification

Bert Popping Bert.Popping at durham.ac.uk
Mon Feb 6 11:09:42 EST 1995


Hi netters,

has anyone ever tried to purify a library?

The story:

I've been sent three diffrent libraries: a genomic library in a 13kb 
vector, a cDNA in a 6kb vector and a genomic library in a 7kb vector.
None of the libraries is very good (more than 50% empty vectors in one case).
(All vectors are E.coli-yeast shuttle vectors)

Is there a protocol for 'purifying' the libraries ?
If 'yes', does one have to use different protocols for the 6/7 and the 
13kb vectors ?

Thanks in advance.


Bert Popping




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