T7 polymerase stable expression in eukaryotic cells?
springer at immsvr.jr2.ox.ac.uk.
Mon Feb 6 16:11:35 EST 1995
Does anybody know about a vector that will stably express T7 polymerase
in human or mouse cells, to a fairly high level? This is to put a gene
of interest into these cells to be expressed from a T7 promoter (safety
considerations), either by vaccinia or by transient transfection.
Of course, we could clone the gene ourselves in pcDNA3... :-)
please e-mail: sebastian.springer at clinical-medicine.oxford.ac.uk
More information about the Methods