T7 polymerase stable expression in eukaryotic cells?

springer springer at immsvr.jr2.ox.ac.uk.
Mon Feb 6 16:11:35 EST 1995

Does anybody know about a vector that will stably express T7 polymerase
in human or mouse cells, to a fairly high level? This is to put a gene
of interest into these cells to be expressed from a T7 promoter (safety
considerations), either by vaccinia or by transient transfection.

Of course, we could clone the gene ourselves in pcDNA3... :-) 

please e-mail: sebastian.springer at clinical-medicine.oxford.ac.uk


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