Cloning small PCR products

Shahram Mori smori at nmsu.edu
Tue Feb 7 02:09:41 EST 1995


Adam S. Asch (asch at mail.med.cornell.edu) wrote:
: Does anybody have any suggestions for increasing the efficiency of
: subcloning short (100-200bp) PCR fragments into a vector such as TA? 
: Thanks.

: Adam Asch, M.D.
: asasch at mail.med.cornell.edu

: -- 
: asasch at mail.med.cornell.edu (Adam S. Asch)

I have heard TA cloning vectors don't work well. People have had better luck
using the strategene's cloning kit that uses a ligase and srf 1 RE in one.
It's a good idea and it works.
Cheers
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003





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