RNA form. gels

jtbrown at osuunx.ucc.okstate.edu jtbrown at osuunx.ucc.okstate.edu
Mon Feb 6 21:04:06 EST 1995


	Does anyone out there have a particular level of formaldehyde
to use in RNA denaturing agarose gels for northern hybridization analysis?
I've heard some different ideas, but no real reasons why.
	My current recipe for a minigel is:
		0.65g agarose
		4.25ml 10x MOPS/EDTA
		34.85ml water
		cool to 50C
		4.4ml stock (37%) formaldehyde
	This yields a 1.5% gel with about 3.75% formaldehyde.  Some suggest
more, some less.  Any ideas?

	
	Justin Brown





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