directly sequence mRNA

Mike Rott mrott at unixg.ubc.ca
Tue Feb 7 16:06:59 EST 1995


In article <3gpisr$1sq at pauling.wadsworth.org>, zhug at news.wadsworth.org
(Guan Zhu) wrote:

> Since there have been many reports by using primer extension of mRNA to 
> determine the transcription starting site of a particular gene, is there 
> any protocol to using the similar primer extension technique to sequence 
> the 5'-end of mRNA directly ? Any responses will be greatly appreciated!
> Best regards!

Yes, this works fairly well if you have sufficient template RNA.  The
method I have used is described in the reference, Ahlquist, P. et al., J.
Mol. Biol. 153, 23-38.

Mike



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