directly sequence mRNA

[Mike Rott]

In article <3gpisr$1sq at pauling.wadsworth.org>, zhug at news.wadsworth.org
(Guan Zhu) wrote:

> Since there have been many reports by using primer extension of mRNA to 
> determine the transcription starting site of a particular gene, is there 
> any protocol to using the similar primer extension technique to sequence 
> the 5'-end of mRNA directly ? Any responses will be greatly appreciated!
> Best regards!

Yes, this works fairly well if you have sufficient template RNA.  The
method I have used is described in the reference, Ahlquist, P. et al., J.
Mol. Biol. 153, 23-38.

Mike