Question on Biotin

rayj at cts.com rayj at cts.com
Tue Feb 7 12:33:19 EST 1995


I am hoping that someone can answer this question.
How does Citrate effect the binding of Biotin to Strepavidin?  I am concerned because citrate can act 
as a chelator much like glycine.  Since glycine is used to elute biotin from strepavidin, will I see a similar 
effect using citrate?

A. Lee Lang
Chromagen
San Diego, CA 92121
(619) 558-1456

" The Main Obstacle to Progress Is Not
Ignorance, BUT The Illusion of Knowledge" 
                            -- Daniel Boorstin




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