PCR Question

Jim Kyriannis jim at phri.nyu.edu
Mon Feb 6 23:50:17 EST 1995


I have 2 30-mer PCR primers which have a Tm higher than 100 degrees.  I
have tried to use these two primers to PCR directly from yeast colonies with no
success.  I have used other primers under the same conditions, which have
worked just fine.  Could the high Tm be the source of the PCR reaction
failures?


Thank you.

Jimmy



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