PCR: PWO DNA polymerase

Ronald Smulders ronsmul at sci.kun.nl
Wed Feb 8 09:40:53 EST 1995


Hi PCR experts,

I'm using a PCR strategy to convert a gene into a cDNA. This strategy
requires several PCR amplifications and therefore I need a reliable
DNA polymerase. I have heard that Boehringer Mannheim sells a so called
PWO DNA Polymerase which has 3'-5' proofreading activity. Furthermore, I have  
heard some rumours that this enzym is better than pfu and vent polymerase.
Does anyone of you have good or bad experiences with PWO? Thanks in advance
for your comments.

Ronald (ronsmul at sci.kun.nl)



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