amylase assay

S. Leangon S.Leangon at massey.ac.nz
Thu Feb 9 02:16:00 EST 1995


Dear netters,

Does  anybody  know  how  to  prove the existance of both alpha-amylase and 
glucoamylase  enzyme in the same enzyme extract ?  (the extract is from a 
fungus cultured on a semi-solid starch-containing substrate).  As much as I 
know the hydrolysis  product from alpha-amylase activity also contains 
glucose which is  the  only  product  from  glucoamylase  activity.  If I 
measure glucose released when incubation starch with the enzyme extract, 
this might not tell.  Any suggestions ?

Thank you in advance.

"S.Leangon at massey.ac.nz"  



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