Ethanol Precipitating of Genomic DNA

Sean David Moore smoore at mail1.sas.upenn.edu
Wed Feb 8 15:02:23 EST 1995


I wouldn't even spin my genomic DNA until I treat is with a subsequent 
enzyme.  I am always proud of complete and intact genomic DNA.  As I prep 
it, I NEVER draw the DNA though a tip.

Also, I spool the DNA, wash in 75% EtOH, and de-spool in 0.5X TE.  I can 
dry most of the EtOH while it is spooled.

Sean





















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      |\_________.._____                                 Sean Moore
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   | =|  |=|     ~~     ||__________========]----        MCP, UPENN, PVAMC
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