Ethanol Precipitating of Genomic DNA
Sean David Moore
smoore at mail1.sas.upenn.edu
Wed Feb 8 15:02:23 EST 1995
I wouldn't even spin my genomic DNA until I treat is with a subsequent
enzyme. I am always proud of complete and intact genomic DNA. As I prep
it, I NEVER draw the DNA though a tip.
Also, I spool the DNA, wash in 75% EtOH, and de-spool in 0.5X TE. I can
dry most of the EtOH while it is spooled.
Sean
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|\_________.._____ Sean Moore
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| =| |=| ~~ ||__________========]---- MCP, UPENN, PVAMC
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