Question on Biotin

Cornelius Krasel krasel at
Wed Feb 8 13:54:36 EST 1995

rayj at wrote:
> I am hoping that someone can answer this question.
> How does Citrate effect the binding of Biotin to Strepavidin?  I am concerned because citrate can act 
> as a chelator much like glycine.  Since glycine is used to elute biotin from strepavidin, will I see a similar 
> effect using citrate?

I'm somewhat confused. At which pH is glycine used to elute biotine from

AFAIK the binding between biotine and streptavidine is not metal-dependent
and hence not affected by chelators.


/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany  */
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/* "Science is the game you play with God to find out what His rules are." */

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