Question on Biotin
krasel at alf.biochem.mpg.de
Wed Feb 8 13:54:36 EST 1995
rayj at cts.com wrote:
> I am hoping that someone can answer this question.
> How does Citrate effect the binding of Biotin to Strepavidin? I am concerned because citrate can act
> as a chelator much like glycine. Since glycine is used to elute biotin from strepavidin, will I see a similar
> effect using citrate?
I'm somewhat confused. At which pH is glycine used to elute biotine from
AFAIK the binding between biotine and streptavidine is not metal-dependent
and hence not affected by chelators.
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de fax: +49 89 8578 3795 */
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