TA Cloning: a skeptic
reichert at bio.bu.edu
Thu Feb 9 16:42:03 EST 1995
This may be a really stupid question but...Why is the TA vector (in whatever
incarnation - Invitrogen, own making) unstable? If you resuspend it in
clean, nuclease-free water or buffer, how does it self-destruct? One view
I read was that the T's come off. How is that possible? Does the phospho-
diester bond hydrolyze spontaneously? Am I totally off my rocker in being
a skeptic of this degradation / instability? Or is this some marketing
gimmick of the manufacturer?
I guess the bottom line is that I'm having a bloody awful time trying to
subclone my PCR products and all this frustration is making me grouchy.
May the PCR and cloning gods smile upon all of us.
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