Q: how to preserve amphipods for later DNA extraction?
VALERIE PARTRIDGE
740271p at axe.acadiau.ca
Thu Feb 9 12:45:01 EST 1995
Does anyone have experience in preserving amphipods for transport, storage,
and eventual DNA extraction (PCR)?
I have access to protocols and primers for fresh-frozen amphipods (though
I'd be most happy to hear other people's suggestions for primers and
extraction protocols), but I haven't found information on how to preserve
and store the critters when freezing them in the grinding buffer isn't
practical -- specifically, for traveling/shipping.
The main criteria are:
1) able to cross international boundaries and trans-oceanic flights
without fuss or muss (i.e., LEGAL and practicable)
2) simple enough that a neophyte (yours truly) can do it in the field
Ability to store the amphipods for long periods at room temperature would
be a plus.
Thanks in advance for any help.
V. A. Partridge
740271p at axe.acadiau.ca
More information about the Methods
mailing list