probe for neuronal house keeping gene?
Martin D. Leach
leach at bu.edu
Thu Feb 9 10:32:03 EST 1995
In article <3hahp9$9kh at epsilon.qmw.ac.uk>, Colin Dolphin
<c.t.dolphin at qmw.ac.uk> wrote:
> Am posting this for a colleague who is working on the effects of
> neurotrophic factors (NGF, FGF, BDNF etc) in neuronal cell cultures. She
> needs a probe for a "house-keeping" gene which is not changed/up-regulated
> in response to the neurotrophic factors. She has tried several common
> probes, eg. actin, citrate synthase but finds the mRNAs are altered by
> treatments. Does anyone have an idea for an alternative ?
Try neuron-specific enolase....you can get antibody form...
alternatively, the promoter, gene and mRNA are published....
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