PCR & spermidine

Duncan Clark Duncan at genesys.demon.co.uk
Sat Feb 11 16:44:35 EST 1995

In article: <Pine.DYN.3.91.950210143021.5038B-100000 at uxa.cso.uiuc.edu>  hodges bradley l <segdoh at uxa.cso.uiuc.edu> 
> You didn't mention if there is an intron(s) between your primer pairs on 
> the genomic DNA.  TAq polymerase can PCR 2kb at the most (in my 
> experience).  So if this true that may be your problem.  

I regularly amplify >3kb without any problems. Even 5kb is not that difficult.



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