HELP. REPROBING BLOTS WITH ECL kit
theano at gecko.biol.wits.ac.za
Sat Feb 11 09:20:56 EST 1995
Does anyone know of the best method of reprobing blots using the ECL kit ?
I need at least five results from each blot, However, I can usually only get
one or two. Amersham claims that blots can be reprobed with the same probe
without stripping the blot of the old one. How can this be ? This has never
worked for me. Can the conventional boiling in 0.1 % SDS method be used to
strip blots, and then reprobe with the ECL kit ? Amersham also claims that
the blot should be soaked in the detection reagent overnight to get rid of
the background from the previous reaction. This also doesn't always work.
Can anyone offer tips or other advice in this regard (other than repeating
the blot from scratch ?)
Thanks in advance.
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