Cloning of murine antibodies
ANDREI.POPOV at afrc.ac.uk
Sun Feb 12 14:31:00 EST 1995
in most B-cells (and hybridomas) Ig genes are rearranged
on both alleles. Additional advantage is that by making cDNA
you have not only 1 genomic copy of your target but millions,
thus you reduce the number of cycles and get less of PCR-errors...
Having lots of sites for the sense primer (i mean leader sequences)
also doesnot improve amplification.
More information about the Methods