Cloning of murine antibodies

ANDREI POPOV ANDREI.POPOV at afrc.ac.uk
Sun Feb 12 14:31:00 EST 1995


Hi Giorgio,

in most B-cells (and hybridomas) Ig genes are rearranged
on both alleles. Additional advantage is that by making cDNA
you have not only 1 genomic copy of your target but millions,
thus you reduce the number of cycles and get less of PCR-errors...
Having lots of sites for the sense primer (i mean leader sequences)
also doesnot improve amplification.

Best wishes
Andrei Popov



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