SSCP messed up by heterodupexes

Michael Kloth mtkloth at students.wisc.edu
Sat Feb 11 21:54:49 EST 1995


> We have started to use SSCP. All gels have heteroduplex product as
> well which weaken single strand bands. We tried NaOH and formamide
> as denaturant, but not methylmercury hydroxide. Is the latter dangerous?

We have used methylmercury hydroxide (which is a neuro toxin)
and have not noticed that it makes much difference.  You might
also want to give ficoll a try, it is safer, cheaper and it 
seems to work slightly better (at least for our specific DNA).

Good luck.
Mike Kloth



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