chemiluminescence detection/western blot

carlos h. pedemonte CPedemonte at UH.EDU
Mon Feb 13 19:13:31 EST 1995

One of the problems with this kind of method is the amount of secondary
antibody. I use ECL. With one of the monoclonals, I have to use a dilution
1/20,000 for the anti-mouse-peroxidase; another way several proteins react.
Thus, one of the possibilities is that you determine which is the lowest
concentrations of secondary antibody that give you a response, and the same
for the primary antibody.

Best, carlos

Carlos H. Pedemonte                     Telephone:  713-743-1211 (office)
Dept. of Pharmacology                               713-743-1228 (lab)
University of Houston                   Fax:        713-743-1229

Houston, TX 77204-5515                  e-mail: pedemonte at

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