Human Quant. PCR cDNA Control

Pat Charmley patcharm at u.washington.edu
Mon Feb 13 15:08:22 EST 1995


Dear Everyone,

I'm looking for a good PCR primer set for a human "structural gene" that 
can be used as an internal control in my cDNA PCRs from various tissues.  
I've read that actin is inducible, and thus I'm not interested.  I've 
heard that GAPDH is a good and stabley expressed gene.  Does anyone have 
a good reference, or personal experience?

Also, I'd prefer that the primer pair not be able to amplify a product 
from genomic DNA, e.g. if it spans a large intron.

Thanks.



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