Human Quant. PCR cDNA Control

Jason Lei leij at sage.cc.purdue.edu
Wed Feb 15 00:18:58 EST 1995


ehovig at radium.uio.no (Eivind Hovig) wrote:
>
> In article <3hq92n$q09 at cc.tut.fi>, bltihi at uta.fi (Timo Hiltunen) wrote:
> > Pat Charmley (patcharm at u.washington.edu) wrote:

<GAPDH for RT-PCR control ..........> 

> ..........
> And consider a 5-fold variation: how will such a variation give
> sensitive results when covaried with often very unreliable
> semiquantitative rtPCR assays like limited cycle number. Not exactly
> precise science...
  ^^^^^^^


So what's the consensus of quantitative RT-PCR these days?  Is competitive
RT-PCR with in vitro made RNA transcript (alterd by point mutation, deletion,
or insertion, etc.) the best in terms of "precise?"



Jason Lei, Ph.D.
_________________________

Horticulture Department
Purdue University		(Standard disclaimer)
West Lafayette, IN 47907
U.S.A.



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