Human Quant. PCR cDNA Control
leij at sage.cc.purdue.edu
Wed Feb 15 00:18:58 EST 1995
ehovig at radium.uio.no (Eivind Hovig) wrote:
> In article <3hq92n$q09 at cc.tut.fi>, bltihi at uta.fi (Timo Hiltunen) wrote:
> > Pat Charmley (patcharm at u.washington.edu) wrote:
<GAPDH for RT-PCR control ..........>
> And consider a 5-fold variation: how will such a variation give
> sensitive results when covaried with often very unreliable
> semiquantitative rtPCR assays like limited cycle number. Not exactly
> precise science...
So what's the consensus of quantitative RT-PCR these days? Is competitive
RT-PCR with in vitro made RNA transcript (alterd by point mutation, deletion,
or insertion, etc.) the best in terms of "precise?"
Jason Lei, Ph.D.
Purdue University (Standard disclaimer)
West Lafayette, IN 47907
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