Three primer PCR mutagenesis

rogers elizabeth erogers at umbc.edu
Tue Feb 14 14:13:34 EST 1995


The reference is the March 1994 Biotechniques, Vol. 16, N0. 3, p.412.
I did it once and got good PCR product but couldn't find my mutation 
after cloning into my vector, though I didn't pursue it rigorously.  I 
also used the standard Perkin-Elmer amounts of primer and nucleotides 
instead of the those in Biotechniques.  Hope it helps.   



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