chemiluminescence detection/western blot

AShaw5 ashaw5 at
Tue Feb 14 21:56:14 EST 1995

1.  Try diluting both primary and secondary antibodies.
2.  Try different blocks like gelatin, BSA, ovalbumin and milk.  I use oa
combination of BSA, ova and milk.
3.  Do all of you antibody steps in block.
4.  Shorten up you incubation times.  If the affinity of your antibody is
strong, it should be all over in 15-30 minutes.
Andrey Shaw

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