H2O2 & superoxide

Chutney a.devitt at bham.ac.uk
Wed Feb 15 08:54:56 EST 1995


In article <3hi8tn$ngt at biovax.biobase.dk>, lho at biobase.dk (Lars Henrik
Oestergaard) wrote:

> Hi,
> 
> As I'm just about to enter a new world - I hope someone is able to
> give me a little advice (usefull references & hints)...
> 
> What I want to do is to measure the amounts of hydrogenperoxide &
> superoxide produced by an oxidase during catalysis!
> 
> One way of doing this is by coupling the oxidase-assay with assays
> of catalase & superoxide-dismutase?
> 
> Have any of you ever tried this? I would also be gratefull for any
> references on these matters. The references don't have to involve
> couples of the catalase/superoxide-dismuatese assays to oxidase, 
> some general articles on how to do these assays would be great!
> 
> Thanks a lot
> 
> Lars H. Ostergaard
> ICMB, University of Edinburgh
> 
> (e-mail: l.h.ostergaard at sms.ed.ac.uk or lho at biobase.dk)

I would advise having a look at the papers of O.T.G.jones.  he has been
doing these assays for years and was very helpful for me.



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