multi-primer PCR screening protocol
smori at nmsu.edu
Tue Feb 14 23:57:12 EST 1995
Frank Baas (antropo) (kotech at amc.uva.nl) wrote:
: In article <D3LGwn.110 at ki.se>, rogbel at ki.se (Roger Belusa kbh) writes:
: > Hi netter,
: > I have some questions to ask. First, when I'm doing a RT-PCR and using
: > the Pfu DNA pol. (Stratagene) I don't get any products but if I use Taq
: > or other polymerases it works. could it be the potassium in the RT-mix
: > that inhibits the Pfu?
: > --
: > Roger.Belusa at kbh.ki.se
: Dear Roger
: Pfu proofreading activity will efficiently degrade your cDNA.
: Taq will not.
: Make new primers!
: Andy G
I don't understand your argument andy. Assuming the primers are specific there
is nothing wrong with using pfu.
Shahram Mori _/\_
Program in Molecular Biology _\ /_
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
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