RT-activity of Taq polymerase, HELP!!!!
salger at wap18.zi.biologie.uni-muenchen.de
Wed Feb 15 17:29:58 EST 1995
Jorge.Hernandez at bota.unine.ch wrote:
: Hello netters!
: When analyzing gene expression by RT-PCR (consult Simpson et al., 1992, NAR
: Vol 20, 5861-5862), I obtained positive signals (bands at the expected size)
: PCR (control) reactions without reverse transcription. It seems that Taq
: merase in certain conditions can exhibit some RT-activity. Does anyone know
: of references concerning this problem? I use DNAse I treated total RNA
: (soybean) and Super Taq polymerase (P.H. Stehelin & Cie AG, Basel).
: All suggestions would be appreciated.
: Thanks in advance,
: E-mail: Jorge.hernandez at bota.unine.ch
I know of two references:
Reverse transcription and direct amplification of cellular RNA
transcripts by Taq polymerase.
Tse WT; Forget BG, Gene 88: 293-6 (1990)
Reverse transcription of mRNA by Thermus aquaticus DNA
Jones MD; Foulkes NS, Nucleic Acids Res 17: 8387-8 (1989)
Klaus Salger phone : ++49 (0)89 5902 -502
Zoologisches Institut FAX : -450
AG MacWilliams e-mail: salger at zi.biologie.uni-muenchen.de
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