Human Quant. PCR cDNA Control

Martin D. Leach leach at bu.edu
Wed Feb 15 10:41:44 EST 1995


> 
> So what's the consensus of quantitative RT-PCR these days?  Is competitive
> RT-PCR with in vitro made RNA transcript (alterd by point mutation, deletion,
> or insertion, etc.) the best in terms of "precise?"
> 
> 
> 
> Jason Lei, Ph.D.
> _________________________

IMHO i believe that is the best internal control......where point mutation
results in a new restriction enzyme site...digestion by this results in a
smaller product (internal standard) and can be compared with the intact
sample product.

Martin

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