Human Quant. PCR cDNA Control
Martin D. Leach
leach at bu.edu
Wed Feb 15 10:41:44 EST 1995
> So what's the consensus of quantitative RT-PCR these days? Is competitive
> RT-PCR with in vitro made RNA transcript (alterd by point mutation, deletion,
> or insertion, etc.) the best in terms of "precise?"
> Jason Lei, Ph.D.
IMHO i believe that is the best internal control......where point mutation
results in a new restriction enzyme site...digestion by this results in a
smaller product (internal standard) and can be compared with the intact
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants
USA on your
My homepage: http://188.8.131.52/Leach.html
Biotech company listing: http://184.108.40.206/jumper/biolcomm.html
p.s. try BioMOO -virtual biology on the internet..
telnet bioinfo.weizmann.ac.il 8888
More information about the Methods