isoamyl alcohol

4700gbera at umbsky.cc.umb.edu 4700gbera at umbsky.cc.umb.edu
Wed Feb 15 09:46:23 EST 1995


In article <3hol30$gla at news.ycc.yale.edu>, burbly at minerva.cis.yale.edu (Marc A. Borbely) writes:
>i have looked everywhere but can't find an answer:
>why in the world is isoamyl alcohol used together with phenol and 
>chloroform for extraction of nucleic acids?

I remember reading somewhere also that it's included to cut down on foaming
and to make the gunk at the interface more compact. I think it does make
a difference but it's been a while since I did an extraction without it, so
my memory may be a bit subjective...

-gb






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