DNA prep for transient transfection

John Storring jstorrin at pharma1.pharma.mcgill.ca
Thu Feb 16 13:44:54 EST 1995


Question,

I am trying to prepare large amounts of clean plasmid for transient
transfection into neuronal cells  with CaHPO4 precipitation. However CsCl
purification gives
me plasmid which doesn't appear to give a high enough efficiency for what I
need.
DNA purified with acid phenol extraction seems to work well but doesn't
give high enough yields (I don't want to do a new maxiprep each time I want
to do a transfection). 
Has anyone got any ideas for what to do? Replies by E-mail please
Thanks
John Storring



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