Beta-galactosidase extraction from tissues?

Dan Settles dan_settles at
Thu Feb 16 11:53:35 EST 1995

Does anyone know of a good method for extracting beta-galactosidase from
animal tissues?  A friend wants to extract it from a transgenic mouse, and
identify it on a western blot with an anti-beta-galactosidase Ab.  So far
no success, even though they "know" the protein is in the tissue.  Thanks.

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