Three primer PCR mutagenesis

[Robert Woodward]

In article <3hoiol$sb9 at delphi.bc.edu> Charlie Hoffman <HOFFMACS at hermes.bc.edu> writes:

>Hi netters,
>Does anyone remember (or better yet has anyone used successfully) a
>site-directed mutagenesis protocol involving PCR with an internal
>phosphorylated primer that carries the mutation.  I think I saw an
>article a year or so ago in Biotechnique, but am having trouble finding
>it again.  Thanks for any help on this.
>Charlie Hoffman
>"I'd rather be fission."

The reference is vol16, no3, page410, 1994.  I haven't tried it myself - a 
straight-forward megaprimer method has worked for me.

--Robert

+--------------------------------------------------+
|  Robert Woodward    c557652 at showme.missouri.edu  |
| Department of Physiology, University of Missouri |
|      voice: 314-882-5374, FAX: 314-884-4276      |
|         http://www.missouri.edu/~c557652         |
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+--------------------------------------------------+
|   Robert Woodward, c557652 at showme.missouri.edu   |
| Department of Physiology, University of Missouri |
|        (314) 882-5374, FAX:(314) 884-4276        |
+--------------------------------------------------+