CAN YOU KEEP PLASMID DNA AT RT?

Michael Cooley szcooley at dale.ucdavis.edu
Tue Feb 14 13:36:14 EST 1995


ARMIN SEPP  (Tel 44+223+832312 Ext547) (armin.sepp at afrc.ac.uk) wrote:
: HI,
: Has anybody of you come across a safe and reliable method for long-term storage
: of plasmid DNA at room temerature?

: My freezer is overflowing and even a new one would only be a temporary
: solution. Therefore, has anybody thought about or experimented with e.g.
: freeze-drying, trechalose, etc. Something that would keep the bugs away and
: would allow to recover the stuff at any time just by transformation.
:  
: armin sepp

: seppa at bbsrc.ac.uk


I have been storing plasmid lypholized at RT. I simply punched a hole in 
the top of the tubes, lypholized, then stored with dessicant.


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