DNA quantification

Chris Seidel seidel at mendel.berkeley.edu
Thu Feb 16 17:27:57 EST 1995


Regarding quantitation of DNA using the ethidium "spot test",
I've had great success with that, but it's essential to make
your standard curve each time, and of course you have to use topologically
similar samples, e.g. compare linear to linear or supercoiled to
supercoiled.

Minigels are an excellent way to compare and quantitiate samples.

Another way that may involve some expense, but not if you can
find someone in your department that has one, is to use a 
fluorimeter. It's a device that takes a spec of your sample bound to
a dye in a very small volume (e.g. a capillary tube) and works
in the ng range.

Chris



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