RT-activity of Taq polymerase, HELP!!!!

Timo Hiltunen bltihi at uta.fi
Fri Feb 17 04:09:48 EST 1995


Klaus.Matthaei at ANU.EDU.AU wrote:
: I think that it is even better to design your primers not only in two
: different exons but IN two exons i.e. ACROSS an intron.  In this way you
: cannot amplify DNA since one half of you primer is in one exon and the
: other half in another.

That is a good principle to follow, but in every case, you must
remember pseudogenes. So, RT- (or RNA) controls should always
be included.
Timo Hiltunen



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