RT-activity of Taq polymerase, HELP!!!!

[Timo Hiltunen]

Klaus.Matthaei at ANU.EDU.AU wrote:
: I think that it is even better to design your primers not only in two
: different exons but IN two exons i.e. ACROSS an intron.  In this way you
: cannot amplify DNA since one half of you primer is in one exon and the
: other half in another.

That is a good principle to follow, but in every case, you must
remember pseudogenes. So, RT- (or RNA) controls should always
be included.
Timo Hiltunen