DNA prep for transient transfection

Martin D. Leach leach at bu.edu
Fri Feb 17 11:13:39 EST 1995


I routinely using Qiagen maxiprep'd dna for transient transfections.

Always check the amount of nicked bands prior to transfection...if there
appears to be a lot (10-15%) then the batch of plasmid is not used for
transfections.

ciao

Martin

no affiliation with qiagen...

-- 
.....          Martin Leach                Email:leach at bu.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants 
               USA                                on your
                                             head.....WIBBLE"
My homepage: http://155.41.115.114/Leach.html
Biotech company listing: http://155.41.115.114/jumper/biolcomm.html

p.s. try BioMOO  -virtual biology on the internet..
telnet bioinfo.weizmann.ac.il 8888
 



More information about the Methods mailing list