TA Cloning

HHOFF at CRCVMS.UNL.EDU HHOFF at CRCVMS.UNL.EDU
Sat Feb 18 14:20:46 EST 1995


We're interested in the method mentioned by a netter recently using
Taq polymerase and dTTP to put a T overhang on your favorite vector 
(to be used in home-made TA cloning).  Unfortunately, I had already deleted
the message by the time I mentioned it to my labmate, who needs more details.
We'd greatly appreciate a more detailed protocol.
Thanks in advance, Heidi



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