Blocking peroxidase in sections

Stacy Ferguson sferguso at kimbark.uchicago.edu
Sat Feb 18 13:29:25 EST 1995


I've been doing a lot of immunohistochemistry lately. Most of my antibodies
work fine after pretreatment of my sections with peroxide in methanol to
destroy endogenous peroxidase activity. One, however, fails to bind after 
this treatment. Does anyone have an alternative method for inhibiting the
activity without destroying my antigen or inhibiting my ABC complex at the 
later steps?


Thanks in advance.

Stacy






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