DNA & RNA RUN ON AGAROSE GELS

Michael Kloth mtkloth at students.wisc.edu
Sat Feb 18 13:06:17 EST 1995


> Well the gel was just started a little while
> ago and (this is the first time I've done this) the appearance is
> unexpected.  We are using bromophenol blue and xylene cyanol (I think on the
> latter).  I expected the stain to simply run ahead of everything else like
> it bromophenol blue does on SDS-PAGE.  What we are observing is the lanes
> with DNA are running faster AND they are seperating into 2 bands.  What am I
> missing here?


Depending on the percent agarose that you are using, the
bromphenol blue dye could run as high as 500 b.p.  If you have
current protocols in molecular biology available, they have
a list of  how fast the dyes run on the different percentage
agarose gels.




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