HELP NEEDED FOR pfu DNA POLYMERASE

Yi, Xiaoming Z3C20 at ttacs3.ttu.edu
Sat Feb 18 23:18:20 EST 1995


I am a using pfu DNA pol(Stratagene) to amplify a 400bp sequence with a 18mer
and a 500bp PCR fragment as the other primerr. I could not get enough product.
Can anybody in this group tell me what I should do? I am using the 10X buffer
with the enzyme and standard conditions.
Any help will be appreciated.



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