buffer for Taq/Pfu polymer's?

Shahram Mori smori at nmsu.edu
Fri Feb 17 21:36:24 EST 1995


gsbs1018 at UTSPH.SPH.UTH.TMC.EDU wrote:
: Hi, 
: 	I am trying to use both Taq (from Perkin Elmer) and Pfu for my PCR
: reactions. What buffer should I used for that reaction? What ratio of this two
: enzyme works better? How is the proof-reading activity? 

: 	Please give me your suggestions, Thanks!

: Song

Perkin Elmer's buffer II. 10X {500mM KCL , 100mM Tris-HCl Ph 8.3.}
Cheers
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003





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