TA cloning

Nagarajan Ramesh ramesh at u.washington.edu
Mon Feb 20 18:08:48 EST 1995

Hi The Nuclec Acids Research reference for the T-vector construction is
NAR Vol: 19, No 5, P1154.


On 17 Feb 1995 HHOFF at CRCVMS.UNL.EDU wrote:

> I told someone in my lab about the use of any blue-white vector with
> EcoRV site for TA cloning by use of dTTP with Taq polymerase, etc.
> Unfortunately, I deleted the original message from my mailbox, but we
> would like more details on methods (although it sounded fairly straight-
> forward).  Thanks in advance, Heidi

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