Kinase questions

Cornelius Krasel krasel at
Mon Feb 20 17:39:11 EST 1995

Neal Gliksman (gliksman at wrote:
> 1.  Does anybody have any references that might indicate that a kinase
> substrate in which the phosphorylated residue has been changed to an
> alanine might have an increased affinity for the kinase or even work as a
> competitive inhibitor of the kinase?

PKCs usually possess a pseudosubstrate site which keeps the kinases
inactive unless they are activated by Ca2++ and/or DAG. This
pseudosubstrate site is similar to a substrate, but it cannot
be phosphorylated since an appropriate residue is missing.


/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany  */
/* email: krasel at                 fax: +49 89 8578 3795  */
/* "Science is the game you play with God to find out what His rules are." */

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