Extracting of cDNA from phage, question

Robert B. Sisk rsisk at med.unc.edu
Mon Feb 20 10:40:34 EST 1995


I have a library packaged in Stratagene's Lambda ZAP Express
vector. Using their ExAssist protocol I have generated Bluescript
phagemid in filamentous phage particles. At this point what I would
like to do is extract the cDNA from the filamentous phage and attempt
to PCR up my sequence of interest. Can anyone suggest a method for
extracting the cDNA directly from the filamentous phage?

Thanks in advance for your help.

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- Robert B. Sisk			      Internet: rsisk at Med.UNC.EDU -
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