pET expression system

Marc Visconti scontz at passport.ca
Tue Feb 21 16:01:25 EST 1995


I would like to know if there is anyone using the pET expression system
from Novogen.  I am currently trying to express and purify a 80 kDa protein
using  pET-30a plasmid in BL21 lys.  In terms of expression, I get
increadible induction, however my yield is very low.  If there is any
tricks that you know I would appreciate it.  Please send all info to :   
scontz at passport.ca       or       marcvis at resunix.ri.sickkids.on.ca   
Thank you 



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