DNA prep for transient transfection

gsbs1018 at UTSPH.SPH.UTH.TMC.EDU gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
Tue Feb 21 15:19:29 EST 1995


Try to extract your dna with only 1/10-1/15 vol. of acid phenol instead of 1:1.
 then add chlorofome , mix again, then spin. Chlorofome will desolved acid
phenol that maybe desolved in solution.Small amount of acid phenol can removed
most of unwanted DNA but will not get rid of most supercoil plasmid that normal
give you low yield.

Good luck!

Song-Kun Shyue  



More information about the Methods mailing list