[Q] Direct sequencing of lambda templates

angelz angelz at salk.sbrc.umanitoba.ca
Tue Feb 21 14:50:47 EST 1995


Dear george:

We perform in routine basis direct DNA sequencing of cDNA and genomic
clones we obtaain from screnings. You need to purify the clone and
amplify a mini-prep. From that source you can perform double strand DNA
Taq polymerase sequencing using the Life Technologies kit. You need
primers complementary the flanking sequence of both arms or a specific
primer to your gene/cDNA of interest.

Dr. Angel Zarain-Herzberg
Div. Card. Sci. 
Univ of Manitoba

angelz at salk.sbrc.umanitoba.ca



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