Plant DNA Isolation

Mail at scri.sari.ac.uk Mail at scri.sari.ac.uk
Wed Feb 22 11:01:21 EST 1995


I have a couple of questions about preps for isolating plant DNA. First, how 
do you get the CTAB and PVPP to dissolve in the CTAB extraction buffer? Or is 
it even supposed to?? 
 
Secondly, would someone post or send me the recipe for the extraction buffer 
used by Dellaporta et al. in their prep in the Maize Handbook. I've used that 
in the past and it worked just fine, so I may switch back. Obviously, I don't 
have my protocol with me.

Thanks!

Nadine Henderson
Scottish Crop Research Institute
fibnh at scri.sari.ac.uk
(ignore the other address on this posting)



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