XYZZYX at DELPHI.COM
XYZZYX at news.delphi.com
Thu Feb 23 00:47:24 EST 1995
Denni Schnapp <ds4 at st-andrews.ac.uk> writes:
>Could anyone enlighten me about the dangers of inhaling 2-mercaptoethanol?
>There seems to be disagreement about how harmful the stuff actually is,
>though certainly everyone agrees it reeks! Also, for this reason I thought
>DTT would be a better alternative for use in SDS PAGE reduction buffers.
>However, it does not seem to be very popular. Has anybody tried it with
>Tricine SDS PAGE?
>Thanks for your advise!
>Denni (ds4 at st-and.ac.uk)
can't comment on the dangers (although it can't be that great for you).
DTT works very well, actually. Works with Tricine gels too. I seem to
recall reading about a phosphorous containing molecule from Pierce that
didn't smell and was supposed to be a good reducing agent to replace
BME. Check the Pierce chemical catalog.
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